Phone: +39-051-6366791
E-mail address: giulia.bottau@ior.it
Bottau Giulia, MSc
CV
Date of Birth
23/03/1995
Qualification
Biotechnologist
Current assignment
Research Scholar
Title of qualification awarded
2022: Master’s degree in Animal Biotechnology at University of Bologna, Class LM-9 Medical, Veterinary and Pharmaceutical Biotechnologies. Thesis: Role of heme oxygenase in ferroptosis induction of osteosarcoma cells (MG-63), graduated with 110/110 cum laude.
Other qualifications awarded
2019: Bachelor’s degree in Animal production and wildlife management, graduated with 101/110. Thesis: Application of nanotechnology in animal reproduction.
Positions held
- July 2022 – Present: Scholarship Fellow at Laboratorio di Patologia delle Infezioni associate all’Impianto of Istituto Ortopedico Rizzoli in Bologna, in the context of the project “Implant orthopedic infections: the challenge of bacterial antibiotic resistance and new anti-infective materials”.
- March 2021 – August 2021: trainee for Master thesis preparation abroad, during Erasmus+ Program, at the Institute for Medical Biochemistry of Vetmeduni Vienna (Austria). Determination of heme oxygenase activity through bilirubin levels determined using a double beam photometer in osteosarcoma cells MG-63 and undifferentiated human bone marrow stem cells (osteoblast like), supplemented with ferroptosis inducing agents. Protein determination through Bradford.
- June 2021 – July 2021: trainee for Master thesis preparation abroad, during Erasmus+ Program, at Ludwig Boltzmann Institute for Traumatology (Vienna, Austria). The studies were focused on osteosarcoma cells MG-63. Cell culture, ferroptosis induction, electron microscopy, determination of lactate dehydrogenase activity in cell culture medium.
- October 2020 – February 2021: student trainee at Laboratorio BCM, Biochimica e Biotecnologie cellulari e molecolari (BCM), Ozzano dell’Emilia (BO). Gene expression of HIF-1α e HIF-2α genes using qPCR and its efficiency evaluation in porcine corpus luteum. Sequencing and amplification of a coriolysin gene fragment in European eel. Gene expression of transferrin receptor gene in canine osteosarcoma. Methods: sampling, agarose gel electrophoresis, preparation of stock PCR primers, PCR-RT, gene expression.
- February 2017 – June 2017: Student Trainee at INFA (Istituto Nazionale di Fecondazione Artificiale).
Languages
English: fluent
French: basic
Technologies
Skilled in Microsoft Office (Word, Excel, PowerPoint) and use of scientific literature database (NCBI, PubMed).
Clinical and/or Scientific interests
Cell culture, ferroptosis and enzymatic activity determination in osteosarcoma cells.
Gene expression.