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Analysis area and methods


Morphological analysis
  • inclusion, section and staining of histological preparations
  • preparation of cytological samples
  • immunohistochemical staining and image analysis.
Cell biology
  • primary cultures from normal and pathological tissues, cultures of normal and transformed cell lines
  • multiple separations of cell populations
  • proliferative activity and cytolytic activity assays
  • transfection of nucleic acids, stable transduction of interfering RNA
  • immunoenzymatic reactions and dosages of soluble factors by multiplex technologies
  • immunofluorescence staining, flow cytometry
  • flow-fish.
Molecular biology
  • PCR (polymerase chain reaction), RT-PCR (reverse transcription-PCR), real-time PCR
  • genomic expression profiling, gene expression scanning
  • western blot
  • in situ hybridization.