Project Technopole (“Tecnopolo”) – Execution of dynamic cultivation through the utilization of custom bioreactors for the realization of engineered constructs for medicine

2003: Master Degree in Pharmacy, University of Bologna.

2009: PhD in “Research methodologies in vascular diseases”, University of Bologna.

• July 2015 – February 2016: Research fellow at RIT-Prometeo laboratory (IOR). Project title: Development of custom bioreactors for dynamic cell culture and engineered tissue substitutes in regenerative medicine.
• March 2015 – June 2015: Support teacher of the course “Biochemistry” at the QuVI department – University of Bologna.
• January 2015 – February 2015: Teacher of Applied Biochemistry 2 at Cesd s.r.l. (Bologna) for students of the University of Bologna (Faculty of Pharmacy).
• April 2012 – December 2014: Post-doctoral research fellow at the Health Science and Technology – Interdepartmental Center for Industrial Research (HST-CIRI), via Tolara di Sopra 50 - 40064 Ozzano Emilia, Italy. Project title: Multi-scale technological devices interacting with cells and molecules.
• April 2011 – March 2012: Post-doctoral research fellow (annual renewal) at the Department of Biochemistry of the University of Bologna. Project title: Dynamic culture of engineering tissue constructs for myocardium regenerative medicine
• April 2009 – 2011: Post-doctoral research fellow at the Department of Biochemistry of the University of Bologna. Project title: Dynamic culture of engineering tissue constructs for myocardium regenerative medicine.
• January 2009 –March 2009: Postdoctoral research fellow at the National Institute for Cardiovascular Researches (INRC) of the University of Bologna.
• January 2006 – December 2008: PhD student in Research methodologies in vascular diseases at the Department of Biochemistry, University of Bologna. Research project: Reparation of the myocardial ischemic damage by mesenchymal stem cells processed by electromechanical stimulation in bioreactor.
• September 2005 – December 2005: Fellowship from the Foundation “Fondazione per lo studio della Fibrosi Cistica” of Verona (Italy). Research
  project: Macrolide antibiotics effects on ionic transport through the CFTR protein. The research was performed at “Molecular and Cellular Engineering Laboratory, Cesena (Italy).
• June 2004 – May 2005: Fellowship granted by INRC at the “G. Moruzzi” Biochemistry Department, University of Bologna. Research project entitled: Physiopathology of ventricular cardiac ipertrophy: rule of the interaction in signal-transduction pathways” dependent on L-arginin.
• April 2001 –September 2001: Professional training useful to practice the pharmacist activity at the Pharmacy “Dell’Immacolata”, Dr. M. Baraldi, Pieve di Cento (Italy).

English: fluent.

• Graphic design and illustration software (Adobe Photoshop, Acrobat Pro);
• Applied statistical analysis (Prism GraphPad);
• Gene expression data analysis (DataAssist Applied Biosystem);
• Protein expression data analysis (QuantityOne Bio-Rad, ImageLab Bio-Rad).

• March 2015 – June 2015: Support teacher of course “Biochemistry” at QuVI department – University of Bologna.
• January 2015 – February 2015: Teacher of Applied Biochemistry at Cesd s.r.l. (Bologna, Italy) for students of University of Bologna (Faculty of Pharmacy).
• May 2007 – December 2014: Member of the examination board of the classes in Biochemistry at the second faculty of engineering of the University of Bologna.

Seminars:

• March 14th, 2014: “Ingegnerizzare il microambiente per indurre differenziamento” at the University of Bologna during the UniStem Day 2014.
• May 14th, 2009: Seminar at the Second Faculty of Engineering - University of Bologna entitled: “Coltura dinamica in bioreattore di cellule staminali mesenchimali da indirizzare verso il fenotipo muscolare cardiaco”.
• October 17th, 2008: Seminar at Dep. of Biochemistry “G. Moruzzi” – University of Bologna entitled: “Riparazione del danno ischemico ostruttivo del miocardio mediante cellule staminali mesenchimali sottoposte a stimolazione elettromeccanica in bioreattore”.

Laboratory practice as part of the course of Biochemistry (prof. E. Giordano) for students of Biomedical Engineering at University of Bologna:

• March 7th 2006: light microscopy, cardiomyocytes and cardiomyoblasts morphology; agarose gel electrophoresis of DNA; Western blotting.
• February 02nd, 2006: light microscopy, cardiomyocytes and cardiomyoblasts morphology; agarose gel electrophoresis of DNA; Western blotting; tripsynenzymatic treatment of H9c2 cell line: the use of workstation Warner.
• February 27th, 2006: light microscopy, cardiomyocytes and cardiomioblasts morphology; patch clamp; SDS-PAGE.
• February 9th, 2005: agarose gel electrophoresis of DNA.

Supervision of students:

Co-supervisor of 7 thesis: n. 4 for bachelor in Biomedical Engineering; n. 2 for bachelor in Motor Science; n. 1 for master degree in Biomedical Engineering.

Participation in research programs:

• April 2011 - December 2014: Multiscale technological devices interacting with cells and molecules POR-FESR (Principal Investigator).
• 2009 - 2011: Dynamic colture of engineering tissue constructs for myocardium regenerative medicine. National Institute for Cardiovascular Diseases (Istituto Nazionale per le Ricerche Cardiovascolari - INRC).
• 2007 - 2009: Dynamic colture of engineering tissue constructs for myocardium regenerative medicine. National Institute for Cardiovascular Diseases (Istituto Nazionale per le Ricerche Cardiovascolari - INRC).

Book chapter:

Dynamic cell culture in cardiac tissue engineering and regenerative medicine. Marco Govoni, Emanuele Giordano, Claudio Muscari and Carlo Guarnieri. “Disputationes: Adult Progenitor Cell Standardization” ed. by P. Di Nardo, River Publishers, Postboks 1657, Algade 42, 9000 Aalborg, Denmark (2012).

Patent:

Patent number: 1395109
Date of patent: 05/09/2012
CCIAA: Milano, Italy
Title: Bioreactor for stem cells

International conferences:

• Sorting single human adult mesenchymal stem cells from lipoaspirates using dielectrophoresis. F. Fontana, F. Bonafè, M. Govoni, A. Doffini, G. Bregola, C. Bolognesi, A. Calanca, S. Gianni, M. Sergio, G. Signorini, P.G. Morselli, C. Guarnieri, C. Muscari, G. Medoro, N. Manaresi, E. Giordano. Quantitative Single Cell Biology in Stem Cell Research, Munich (Germany), Oct. 10th-11th 2013, poster n. 15.
• FoodOmics for discovering the mechanism of action of dill and kale extracts on cholesterol homeostasis and metabolism. F. Danesi, M. Govoni, D. de Biase, L. F. D’Antuono, M. E. Woodcock,  A. Konić-Ristic, M. Glibetić, A. Bordoni. 3rd International Conference on FoodOmics, Cesena (Italy), May 22nd-24th 2013, p. 53-54. ISBN: 978-88-902152-3-0.
• An innovative stand-alone bioreactor for the highly-reproducible transfer of cyclic mechanical stretch to stem cells cultured in a 3D scaffold.  M. Govoni, F. Lotti, L. Biagiotti, M. Lannocca, G. Pasquinelli, S. Valente,  C. Muscari,  F. Bonafè, C.M. Caldarera, C. Guarnieri, S. Cavalcanti, E. Giordano. 1st Scientific Workshop on Stem Cells for Ligament and Tendon Regeneration, Pescara (Italy),  April 18th 2013.
• Hypocholesterolemic effect of dill and kale extracts: assessing mechanisms of action in hepatic cultured cells. F. Danesi, M. Govoni, D. de Biase, S. Saha, M.E. Woodcock, A. Konić-Ristić, M. Glibetić, P.A. Kroon, A. Pession, L.F. D’Antuono, E. Giordano, A. Bordoni. Traditional Food International congress, Cesena (Italy), Oct. 4th-5th 2012. ISBN 978-88-902152-1-6.
• Highly-reproducible transfer of cyclic mechanical stretch to stem cells: a novel bioreactor. M. Govoni, F. Lotti, L. Biagiotti, M. Lannocca, C. Muscari, F. Bonafè, G. Pasquinelli, S. Valente, C.M. Caldarera, C. Guarnieri, S. Cavalcanti, E. Giordano. American Society of Cell Biology, Denver (CO), USA, Dec. 3rd-7th 2011, abstract n. 1337.
• Epigenetic status of cardiac master genes in native adipose derived stem cells. A. Pasini, F. Bonafè, E. Fiumana, M. Govoni, C. Guarnieri, P.G. Morselli, C.M. Caldarera, C. Muscari, E. Giordano. American Society of Cell Biology, Denver (CO), USA, Dec. 3rd-7th, 2011, abstract n. 1719.
• A novel stand-alone bioreactor for the cyclic mechanical stretching of cultured cells. M. Govoni, E. Giordano, C. Muscari, S. Valente, G. Pasquinelli, S.C.M. Caldarera, C. Guarnieri. Nanotechitaly 2011, Venice (Italy), Nov. 23rd-25th 2011, p. 323.
• Epigenetic profiling of native adipose-derived stem cells to be differentiated towards the cardiac phenotype. A. Pasini, F. Bonafè, E. Fiumana, M. Govoni, C. Guarnieri, P.G. Morselli, C.M. Oranges, C.M. Caldarera, C. Muscari, E. Giordano. International Society for Cellular Therapy, Montesilvano (Italy), June 10th-12th 2011, p. 37.
• Bioreactor for stem cells. M. Govoni. ESOF 2010, European Research & Business speed dating catalogue, Turin (Italy), July 6th 2010, p. 212.
• H9c2 interaction with PLLA nanofibrous scaffold. S. Valente, L. Foroni, M.L. Focarete, C. Gualandi, M. Govoni, C. Gamberini, E. Giordano, G. Pasquinelli. Human Pluripotent Stem Cell Symposium, Dublin, Ireland, Apr. 22nd-24th 2009.
• Electrospun bioresorbable nanofibrous scaffolds for tissue engineering applications. M.L. Focarete, M. Scandola, C. Gualandi, A. Zucchelli, F. Lotti, G. Pasquinelli, M. Govoni, C. Gamberini, P. Wilczek. Nanotech 2008, Venice (Italy), March 10th-14th 2008, p. 62.
• A bioreactor for electromechanical stress of cells to address towards cardiac phenotype. F. Lotti, G. Vassura, C. Melchiorri, L. Biagiotti, C. Muscari, E. Giordano, M. Govoni, C.M. Caldarera, C. Guarnieri, S. Cavalcanti. XIX World Congress of the ISHR, Bologna (Italy), June 22nd-25th 2007, p. S89. ISBN: 0022-2828(200706)42:6S;1-Q.
• A bioreactor for electromechanical stress of cells to address towards the cardiac phenotype F. Lotti, G. Vassura, C. Melchiorri, L. Biagiotti, C. Muscari, E. Giordano, M. Govoni, C.M. Caldarera, C. Guarnieri, S. Cavalcanti. Biomed. Pharmacother. 2006. 60(8):469.

Oral communications:

• Coltura dinamica in bioreattori per il differenziamento di cellule staminali per la medicina rigenerativa del miocardio. M. Govoni, E. Giordano, C. Muscari, C.M. Caldarera, C. Guarnieri. Interazioni molecolari tra cellule staminali e superfici polimeriche nello studio della riparazione del miocardio infartuato, Ferrara (Italy), Nov. 18th 2011, p. 39-41.
• A novel bioreactor to mechanically stress rat Mesenchymal Stem Cells (rMSC) in culture. M. Govoni, E. Giordano, C. Muscari, G. Pasquinelli, S. Cavalcanti, C.M. Caldarera, C. Guarnieri. Disputationes-Adult Progenitor Cell Standardization, Rome (Italy), Dec. 1st-3rd 2010, p. 24-25. ISBN: 978-87-92329-54-7.

National conferences:

• Effetto ipocolesterolemizzante di estratti di aneto e di cavolo da foglia: un approccio nutrigenomico. A. Bordoni, M. Govoni, D. de Biase, S. Saha, M.E. Woodcock, A. Konić-Ristić, M. Glibetić, P.A. Kroon, A. Pession, Nadiya Boyko, Bike Kocaoglu, Osman Hayran, L.F. D’Antuono, E. Giordano, F. Danesi. 35° Congresso Nazionale Società Italiana di Nutrizione Umana (SINU) “LARN: Livelli di Assunzione di Riferimento di Nutrienti e Energia per la popolazione  italiana, revisione 2012” Bologna (Italy), Oct. 22nd-23rd 2012.
• Epigenetica delle cellule staminali del tessuto adiposo da impiegare per la rigenerazione del miocardio infartuato. A. Pasini, F. Bonafè, M. Govoni, C. Guarnieri, P.G. Morselli, C.M. Caldarera, C. Muscari, E. Giordano. Interazioni molecolari tra cellule staminali e superfici polimeriche nello studio della riparazione del miocardio infartuato, Ferrara (Italy), Nov. 18th 2011, p. 29-31.
• Transcriptional layout of cardiac master gene promoters in native human Adipose Derived Stromal Cells. A. Pasini, F. Bonafè, E. Fiumana, M. Govoni, C. Guarnieri, P.G. Morselli, C.M. Caldarera, C. Muscari, E. Giordano. XVIII Congresso Nazionale della Società Italiana di Ricerche cardiovascolari (SIRC), Imola (Italy), Oct. 21-22 2011, p. 26.
• Biopolimeri micro e nanostrutturati ingegnerizzati con cellule staminali per applicazioni cardiovascolari. L. Foroni, S. Valente, C. Gualandi, M.L. Focarete, M. Govoni, E. Giordano, G. Pasquinelli, A. Stella. La ricerca vascolare: il punto sulla ricerca nelle malattie vascolari in Italia, Bologna, Oct. 26th 2010.
• Effetti della stimolazione meccanica di cellule staminali mesenchimali in biorattore. M. Govoni, F. Bonafè, E. Giordano, C. Muscari, M. Lannocca, F. Lotti, S. Valente, G. Pasquinelli, C.M. Caldarera, C. Guarnieri, S. Cavalcanti. XVII Congresso Nazionale della Società Italiana di Ricerche cardiovascolari (SIRC), Imola (Italy), Oct. 7th-9th 2010, p. 89.
• A novel bioreactor for a controlled mechanical cyclic stress of stem cells. M. Govoni, F. Bonafè, E. Giordano, C. Muscari, M. Lannocca, F. Lotti, S. Valente, G. Pasquinelli, S. Cavalcanti, C.M. Caldarera, C. Guarnieri. SIB, Milan (Italy), Sept. 14th-17th 2010, p. 136. 
• A novel bioreactor to mechanically stress stem cells in culture. M. Govoni, F. Lotti, M. Lannocca, C. Muscari, E. Giordano, F. Bonafè, G. Pasquinelli, S. Valente, C. Guarnieri, C.M. Caldarera, S. Cavalcanti. Secondo Congresso Nazionale di Bioingegneria, Turin, July 8th-10th 2010, pp. 367-368. ISBN:978-88-555-3082-8.
• Effect of ethanol treatment on Poly(L)lactic acid electrospunfibre morphology. C. Gualandi, M.L. Focarete, M. Govoni, E. Giordano, L. Foroni, S. Valente, G. Pasquinelli. Congresso Nazionale Biomateriali SIB 2010, Camogli (Italy), May 24th-26th 2010, p. 86.
• New biodegradable polyesters for soft tissue engineering. M.L. Focarete, C. Gualandi, M. Soccio, N. Lotti, M. Govoni, E. Giordano, L. Foroni, S. Valente, G. Pasquinelli. Congresso Nazionale Biomateriali SIB 2010, Camogli (Italy), May 24th-26th 2010, p. 82.
• Poly(L-lactic) acid electrospun nanofibrous scaffolds for cardiac tissue engineering. C. Gamberini, M. Govoni, E. Giordano, C. Gualandi, M.L. Focarete, M. Scandola, L. Foroni, S. Valente, G. Pasquinelli, C. Guarnieri. XV Congresso Nazionale della Società Italiana di Ricerche cardiovascolari (SIRC), Imola (Italy), Oct. 9th-11th, 2008, p. 126.
• Nanostructured electrospun three-dimensional scaffolds for tissue engineering applications. M.L. Focarete, M. Scandola, C. Gualandi, A. Zucchelli, F. Lotti, G. Pasquinelli, M. Govoni, C. Gamberini, C. Guarnieri, E. Giordano, C. Muscari, L. Foroni, S. Valente, N. Lotti, M. Soccio. Congresso Nazionale di Bioingegneria, Pisa (Italy), July 3rd-5th 2008, p. 713-714.
• Ruolo delle poliammine nel differenziamento in sensomiogenico di cardiomioblasti ventricolari di ratto. F. Bonavita, M. Govoni, L. Shantz, E. Giordano, C.M. Caldarera, C. Guarnieri. XIV Congresso Nazionale della Società Italiana di Ricerche cardiovascolari (SIRC), Imola (Italy), Sept. 27th-29th 2007, p. 110.
• Monitoraggio digitale della pressione di perfusione del cuore di ratto durante la procedura di isolamento dei cardiomiociti. A. Ciandrini, M. Govoni, E. Giordano, S. Cavalcanti. XIII Congresso Nazionale della Società Italiana di Ricerche cardiovascolari (SIRC), Imola (Italy), Sep. 21st-23rd 2006, p. 113.
• Realizzazione di un dispositivo per lo stress elettromeccanico di cellule staminali mesenchimali in coltura. F. Lotti, G. Vassura, C. Melchiorri, L. Biagiotti, C. Muscari, E. Giordano, M. Govoni, F. Bonafè, M. Carboni, C.M. Caldarera, S. Cavalcanti. XIII Congresso Nazionale della Società Italiana di Ricerche cardiovascolari (SIRC), Imola (Italy), Sept. 21st-23rd, 2006, p. 114.
• Macrolides antibiotics and ion transport across plasma membrane. S. Furini, M. Govoni, E. Grandi, S. Severi, F. Lodesani, M. Bennati, I. Olla, M. Tartagni, P. Iorio, A. Miano, C.M. Caldarera, S. Cavalcanti, E. Giordano. XIII Congresso Nazionale della Società Italiana di Ricerche cardiovascolari (SIRC), Imola (Italy), Sept. 21st-23rd 2006, p. 116.
• Bicarbonato-dialisi verso biofiltrazione in assenza di acetato nell’introduzione della sintesi dell’ossido nitrico in cardiomiociti di ratto. M. Govoni, E. Grandi, S. Furini, S.Severi, V. Mazzone, M. Carboni, A. Santoro, S. Cavalcanti. E. Giordano. Congresso Nazionale della Società Italiana di Ricerche cardiovascolari (SIRC), Imola (Italy), Sept. 21st-23rd 2006, p. 117.
• Realizzazione di un bioreattore per la stimolazione elettromeccanica di cellule staminali mesenchimali in coltura. F. Lotti, G. Vassura, C. Melchiorri, L. Biagiotti, C. Muscari, E. Giordano, M. Govoni, C.M. Caldarera, S. Cavalcanti. Biopolimeri ingegnerizzati con cellule staminali autologhe: terzo workshop delle unità operative, Turin (Italy), Mar. 24th-25th 2006, p. 29.
• Multicharged ornithine decarboxylase species result from post-translational modification upon targeted overexpression in mouse heart. G. Agnetti, L.M. Shantz, K. Bezstarosti, D. Dekkers, M. Govoni, C. Guarnieri, C.M. Caldarera, A.E. Pegg, J.M. Lamers, E. Giordano. XII Congresso Nazionale della Società Italiana di Ricerche cardiovascolari (SIRC), Imola (Italy), Sept. 22nd-24th 2005, p. 110.

Selected partecipation in specialized courses:

• Sept. 16th-19th 2013: XXII Scuola di Bioingegneria: Approccio integrato alla medicina rigenerativa (Bressanone – BZ Italy; duration: 4 days);
• June 18th 2013: Droplet Digital PCR (Bologna; duration: 4 h);
• May 11th 2012: Protein expertise Roadshow 2012 (Bologna; duration: 7 h);
• October 20th 2011: Nano democracy: la qPCR per tutti (Bologna; duration: 7 h);
• May 27th 2010: Learn more about Human Primary Cells–Cell culture reagents and Mycoplasma prevention -“Explore the cell" (Bologna; duration: 3 h);
• September 19th 2009: Victor X Day (Bologna; duration: 4 h);
• June 2008: Real Time PCR: (Bologna; duration: 4 h).

Panel referees activity:

• Tissue Engineering Part A (Mary Ann Liebert Inc, ISSN 1937-3341);
• Journal of Tissue Engineering and Regenerative Medicine (John Wiley and Sons Ltd, ISSN1932-6254).

Regenerative medicine, cell therapy, tissue engineering, adult stem cells, bioreactors and multi-scale technological devices interacting with cells and molecules, biocompatible polymers, cell differentiation.